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Reactive oxygen species (ROS) production by PBMCs stimulated with K. <t>pneumoniae</t> (A) or S. marcescens (B). Percentage of CD11b+ cells positive for DTAF (FITC)-labelled bacteria (C and E) and representative histograms (D and F) for K. pneumoniae and S. marcescens . n=6-9 biological replicates from 2-3 independent experiments, *p≤0.05, **p≤0.01, ****p≤0.0001 Friedman test followed by Dunn’s multiple comparison test. Unst: unstimulated, M9: minimal media, AUC: area under curve as a ratio of ROS production over time, UV: ultra-violet.
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Reactive oxygen species (ROS) production by PBMCs stimulated with K. pneumoniae (A) or S. marcescens (B). Percentage of CD11b+ cells positive for DTAF (FITC)-labelled bacteria (C and E) and representative histograms (D and F) for K. pneumoniae and S. marcescens . n=6-9 biological replicates from 2-3 independent experiments, *p≤0.05, **p≤0.01, ****p≤0.0001 Friedman test followed by Dunn’s multiple comparison test. Unst: unstimulated, M9: minimal media, AUC: area under curve as a ratio of ROS production over time, UV: ultra-violet.

Journal: bioRxiv

Article Title: Immune responses to human pathogens exposed to simulated Mars conditions

doi: 10.1101/2025.02.05.636683

Figure Lengend Snippet: Reactive oxygen species (ROS) production by PBMCs stimulated with K. pneumoniae (A) or S. marcescens (B). Percentage of CD11b+ cells positive for DTAF (FITC)-labelled bacteria (C and E) and representative histograms (D and F) for K. pneumoniae and S. marcescens . n=6-9 biological replicates from 2-3 independent experiments, *p≤0.05, **p≤0.01, ****p≤0.0001 Friedman test followed by Dunn’s multiple comparison test. Unst: unstimulated, M9: minimal media, AUC: area under curve as a ratio of ROS production over time, UV: ultra-violet.

Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.

Techniques: Bacteria, Comparison

Cytokine production following stimulation of PBMCs for 24 hours (A, E) and 7 days (B, F) with K. pneumoniae (A, B) or S. marcescens (E, F). Reactive oxygen species production when stimulated with K. pneumoniae (C) or S. marcescens (G). Phagocytosis of K. pneumoniae (D) and S. marcescens (H) by CD11b+ cells n=6 biological replicates from 2 independent experiments. *p≤0.05, **p≤0.01, Friedman test with Dunn’s multiple comparison test, M9: minimal media.

Journal: bioRxiv

Article Title: Immune responses to human pathogens exposed to simulated Mars conditions

doi: 10.1101/2025.02.05.636683

Figure Lengend Snippet: Cytokine production following stimulation of PBMCs for 24 hours (A, E) and 7 days (B, F) with K. pneumoniae (A, B) or S. marcescens (E, F). Reactive oxygen species production when stimulated with K. pneumoniae (C) or S. marcescens (G). Phagocytosis of K. pneumoniae (D) and S. marcescens (H) by CD11b+ cells n=6 biological replicates from 2 independent experiments. *p≤0.05, **p≤0.01, Friedman test with Dunn’s multiple comparison test, M9: minimal media.

Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.

Techniques: Comparison

TEM visualization of K. pneumoniae (A) and S. marcescens (C), size (FSC) and complexity (SSC) quantification by flow cytometry of K. pneumoniae (B) and S. marcescens (D). Scale bars are 500 nm in panel A and 250 nm in panel C. n=5-7 biological replicates from 2 independent experiments. *p≤0.05, **p≤0.01 Friedman test followed by Dunn’s multiple comparison test, M9: minimal media, UV: ultra-violet.

Journal: bioRxiv

Article Title: Immune responses to human pathogens exposed to simulated Mars conditions

doi: 10.1101/2025.02.05.636683

Figure Lengend Snippet: TEM visualization of K. pneumoniae (A) and S. marcescens (C), size (FSC) and complexity (SSC) quantification by flow cytometry of K. pneumoniae (B) and S. marcescens (D). Scale bars are 500 nm in panel A and 250 nm in panel C. n=5-7 biological replicates from 2 independent experiments. *p≤0.05, **p≤0.01 Friedman test followed by Dunn’s multiple comparison test, M9: minimal media, UV: ultra-violet.

Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.

Techniques: Flow Cytometry, Comparison